Generator
pBAD:CspA

Part:BBa_K538303

Designed by: Bas Stringer   Group: iGEM11_Amsterdam   (2011-09-08)

pBAD + RBS + CspA

pBAD:CspA
K538303


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the CspA protein from Polaribacter irgensii (BBa_K538003) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD (BBa_K206000). E. coli expressing this protein experience a drastic increase in their ability to survive freezing and thawing. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.


Refer to the main page of the coding region (CspA: BBa_K538003) for more information on how CspA facilitates cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//classic/generator/prc
//function/regulation/transcriptional
//regulation/positive
Parameters
functionProtein expression
outputCspA
positive_regulatorsL-arabinose